Discrete Cross-Reactive Epitopes for Flavivirus Serological Diagnostics and Vaccines


CDC researchers have identified and characterized discrete flavivirus cross-reactive epitopes useful for improving serodiagnosis and vaccination of flaviviruses, such as dengue virus, yellow fever virus, Japanese encephalitis virus, West Nile virus and the tick-borne encephalitis viruses.

The flavivirus envelope glycoprotein is one of the primary antigens inducing protective immunity in a host. Human flavivirus infections stimulate virus species-specific as well as flavivirus-genus cross-reactive immune responses. These cross-reactive antibodies create a serious problem for serodiagnosis, especially for secondary flavivirus infections, due to the difficulty of differentiating primary from secondary cross-reactive serum antibodies. Additionally, the presence of subneutralizing levels of flavivirus cross-reactive serum antibodies in an individual may result in a dramatic increase in the severity of secondary flavivirus infections via antibody-dependent enhancement (ADE), as commonly seen with multi-serotype dengue virus infection.

To that end, the identification and characterization of amino acids incorporated into these flavivirus cross-reactive epitopes extends practical understanding of structure-function relationships important for flavivirus immunopathology. This CDC antigen-epitope technology provides important tools and insights for improving flavivirus serodiagnosis, researching the pathogenesis of ADE, and advancing the development of safe and effective flavivirus vaccines.

Potential Commercial Applications: Competitive Advantages:
  • Flavivirus vaccine development
  • Improved serological diagnostics for flaviviruses such as dengue, yellow fever, Japanese encephalitis, West Nile and the tick-borne encephalitis viruses
  • Research of flavivirus-related antibody-dependent enhancement (ADE) pathologies
 
  • Technology circumvents flavivirus serodiagnostic issues associated with cross-reactive antibody detection and non-specific flavivirus infection diagnoses
  • May provide unique solutions for development of flavivirus-genus or species-specific vaccines


Development Stage:
  • In vitro data available


Inventors:

Gwong-Jen Chang (CDC)  ➽ more inventions...

Wayne Crill (CDC)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 7,906,292 issued 2011-03-15
U.S. Pat: 9,000,141 issued 2015-04-07
US Application No. 11/572,805
US Application No. 12/892,714
US Application No. 14/659,251
Various international patents pending and issued

Publications:
Crill WD, et al. PMID 15564505
Chang GJ, et al. PMID 12620809

Licensing Contact:
Tara Kirby, Ph.D.
Email: tara.kirby@nih.gov
Phone: 240-669-5128

OTT Reference No: E-333-2013/0
Updated: May 15, 2014