Real-time PCR Multiplex Assay for Detection of Bacterial Respiratory Pathogens in Clinical Specimens


CDC researchers have developed a single-tube, real-time PCR assay for the simultaneous detection of three bacterial respiratory pathogens (Mycoplasma pneumoniae, Chlamydiophila pneumoniae and Legionella spp.). The assay has an internal control testing for presence of human DNA. This four-plex real-time PCR assay could potentially become a routine screening test for patients with respiratory illness. Ninety four clinical specimens (in a 96-well format) can be tested at once. This assay is non-invasive, rapid and cost-effective. It has the potential for point-of-care applications in population-based pneumonia surveillance.

Potential Commercial Applications: Competitive Advantages:
  • Population-based pneumonia surveillance
  • Development of broadly-capable respiratory clinical diagnostics
 
  • Sensitive and specific
  • High-throughput friendly
  • Rapid and cost-effective compared to screening for individual respiratory pathogens
  • Easily developed for use in diagnostic kits


Development Stage:
  • Pre-clinical
  • In vitro data available


Inventors:

Jonas Winchell (CDC)  ➽ more inventions...

Agnes Warner (CDC)  ➽ more inventions...

Kathleen Thurman (CDC)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 9,260,762 issued 2016-02-16
US Application No. 61/324,986
US Application No. 13/641,444

Publications:
Thurman KA, et al. PMID 21397428

Licensing Contact:
Karen Surabian, J.D., M.B.A.
Email: karen.surabian@nih.gov
Phone: 301-594-9719

OTT Reference No: E-300-2013/0
Updated: Sep 19, 2013