Diagnostic Assays Utilizing Real-Time Taqman or Seminested RT-PCR for Parechovirus Detection and Discrimination


The CDC developed a real-time reverse transcription polymerase chain reaction (RT-PCR) Taqman assay and an RT-semi nested PCR (RT-snPCR) assay for the detection of parechoviruses. Similar to enteroviruses, parechoviruses are responsible for gastrointestinal, respiratory and central nervous system infections. All tests target conserved regions in the 5'-nontranslated region (5-'NTR) of the parechovirus genome and share forward and reverse primers. The Taqman probe and RTsnPCR nested primer target the same conserved site but vary in length. Both assays detect all known human parechoviruses (PPeV) and Ljungan viruses (LV), unlike other published parechovirus 5'-NTR assays, which only detect a limited number of PPeV types. Both assays are more sensitive than current methods (culture and multiple, single-serotype nucleic acid amplification assays) and may be used to test isolates or original clinical specimens.

Potential Commercial Applications: Competitive Advantages:
  • Diagnostic detection of all known species of Parechovirus from clinical samples, including Human parechovirus and Ljungan virus
  • Discrimination of specific species and serotypes
  • Public health surveillance programs
  • Research tool for all lab strains and clinical isolates of parechovirus
 
  • Detects all Parechovirus genus members with a single assay
  • Rapid, accurate, sensitive and specific
  • Cost-effective in terms or resource-input, labor and turnaround time
  • Does not require culturing
  • Easily adaptable to kit form


Development Stage:
  • Early-stage
  • In vitro data available


Inventors:

William Nix (CDC)  ➽ more inventions...

Mark Oberste (CDC)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 8,048,630 issued 2011-11-01
US Application No. 12/299,097

Collaboration Opportunity:

The Centers for Disease Control and Prevention (CDC) is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate or commercialize Diagnostic Assays Utilizing Real-Time Taqman or Seminested RT-PCR for Parechovirus Detection and Discrimination. For collaboration opportunities, please contact Suzanne Shope at sshope@cdc.gov or 770-488-8613.


Licensing Contact:
Karen Surabian, J.D., M.B.A.
Email: karen.surabian@nih.gov
Phone: 301-594-9719

OTT Reference No: E-295-2013/0
Updated: Sep 19, 2013