mNFHcre Transgenic Mice


Knockout mouse is a valuable model to study biological functions of target genes. When Cre expressing mice are bred with mice containing a loxP-flanked gene, the gene between the loxP sites will be deleted in the offsprings. Scientists at the NIH have generated mNF-H-cre transgenic mouse lines that express Cre recombinase under the control of the promoter of the neurofilament-H gene, which is expressed in the late stage of neuronal maturation. The transgenic mice express cre in neurons (but not astrocytes) with highest expression in the cortex and hippocampus. The mNF-H-cre transgenic mouse line can be used to generate conditional knockout mice with targeted excision of neuron-specific genes during the late stage of mouse development. This mouse model will be useful for the study of neuronal functions of particular genes.

Potential Commercial Applications: Competitive Advantages:
  • Generating conditional knockout mice for neurobiological, neuro-developmental, or aging studies involving neurons of the brain and the spinal cord.
 
  • Transgenic mice express Cre recombinase selectively in neurons (but not in astrocytes) in the late stage of brain development.


Development Stage:
In vivo data available (animal)

Inventors:

Ashok Kulkarni (NIDCR)  ➽ more inventions...


Intellectual Property:
Research Tool – Patent protection is not being pursued for this technology.

Publications:
Hirasawa M, et al. PMID 11377750
Hirasawa M, et al. PMID 15067135

Collaboration Opportunity:

The National Institute for Dental and Craniofacial Research is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate or commercialize knockout mice for neurobiological studies. For collaboration opportunities, please contact David Bradley, Ph.D. at bradleyda@nidcr.nih.gov.


Licensing Contact:
David Bradley,
Email: bradleyda@nidcr.nih.gov
Phone: 301-402-9242

OTT Reference No: E-293-2009-0
Updated: Aug 10, 2015