Method for Finding Usable Portion of Sigmoid Curve (the Taylor Method), Improved Assay Readouts, and Enhanced Quality Control/Assurance


CDC researchers have developed algorithmic methods for determining sigmoid curve optimums and calculating component concentrations. Sigmoid curves are commonly generated in bioassays and used to calculate results. Various techniques have been used to define the curve, analyze the observations, and calculate a concentration. This technology is an algorithmic approach to identifying the usable portion of a sigmoid curve. This approach is more objective than other methods, reducing the variability introduced by individuals and/or by repetition and allows substantially higher throughput in a situation where a lot of samples are being analyzed using the same assay.

Potential Commercial Applications: Competitive Advantages:
  • Observation and data analysis
  • Determining concentrations
  • Improving calculations and estimations
  • Enhancing consistency and reproducibility of outcomes for bio and chem assays
 
  • Less output-data subjectivity than alternate methods
  • Rapid, accurate and simple to implement
  • Quality control and assurance for a number of assays such as PCR, ELISA, toxin neutralization assays (TNA), flow cytometry, cell death assays, titrations, etc.
  • Reduces data variability due to errant input
  • Easily adapted to high-throughput analyses
  • Demonstrated efficacy quantifying anthrax lethal toxin neutralization activity


Development Stage:
In vitro data available

Inventors:

Thomas Taylor (CDC)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 7,469,186 issued 2008-12-23
PCT Application No. PCT/US2004/008566
US Application No. 10/550,377
Various international patent applications pending or issued

Publications:
Li H, et al. PMID 18304568

Licensing Contact:
Karen Surabian, J.D., M.B.A.
Email: karen.surabian@nih.gov
Phone: 301-594-9719

OTT Reference No: E-270-2013/0
Updated: Jan 16, 2014