Genome Wide DNase I Hypersensitive Sites Detection in Formalin-Fixed Paraffin-Embedded Single Cells


A method of detecting DNase I hypersensitive sites ((DHS) in a single cell or very small number of cells, including cells recovered from formalin-fixed paraffin-embedded (FFPE) tissue slides of patient samples. DHS has revealed a large number of potential regulatory elements for transcriptional regulation in various cell types. The application of DNase-Seq techniques to patient samples can elucidate pathophysiological mechanisms of gene function in a variety of diseases as well as provide potentially important diagnostic and prognostic information. Unfortunately, the current DNase-Seq techniques require large number of cells and are applicable only to larger biopsies and surgical specimens. This technique, called Pico-Seq, allows detection when only very small population of cells are available, such as rare primary tumor cells and circulating-tumor-cells, isolated by a variety of methods. Pico-Seq uses conditions capable of restoring the DNase I sensitivity, similar to native/fresh cells, in tissue/cells from slides processed by extremely harsh conditions, such as in FFPE tissues.

Potential Commercial Applications: Competitive Advantages:
  • Diagnostic and prognostic kits
  • Research kits
 
  • Applicable to very small number of cells down to a single cell.
  • Capable of using cells isolated by any of the available methods, including flow cytometry, biopsies, laser capture microdissection, and even cells recovered from formalin-fixed paraffin-embedded tissue slides of patient samples.


Development Stage:
  • Early-stage
  • In vitro data available


Inventors:

Keji Zhao (NHLBI)  ➽ more inventions...

Qingsong Tang (NHLBI)  ➽ more inventions...


Intellectual Property:
US Application No. 62/118,574

Licensing Contact:
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OTT Reference No: E-254-2014-0
Updated: Aug 17, 2015