Device to Measure Muscle Contractile-Relaxant and Epithelial Bioelectric Responses of Perfused, Intact Tracheal Airways Tissue In Vitro


CDC and collaborative researchers have developed a device allowing for simultaneous measurement of smooth muscle contractile/relaxant activity and transepithelial potential difference (Vt) [or short circuit currents (Isc)] and resistance (Rt) within an intact airway in vitro. Investigation of the underlying mechanisms of lung diseases, such as asthma or cystic fibrosis, involves understanding the roles of airway smooth muscle and epithelium. Smooth muscle is involved in the control of the airway diameter; epithelium regulates the ionic composition of the liquid lining the airways through electrogenic ion transport and releases factors that regulate the ability of smooth muscle to contract.

This invention allows for the measurement and study of pulmonary diseases under conditions retaining normal spatial relationships between all the cell types and an unmanipulated/undistorted tracheal airway wall. Further, the device permits evaluation of epithelial functional integrity using pharmacological techniques. Agents can be separately added to the lumen, where they must first cross the epithelium to reach the smooth muscle, or to the outside of the airway, where there is no hindrance of said agents to the muscle. The invention also permits the effective in vitro screening of the effects of agents and drugs on airway epithelium and smooth muscle within the same preparation.

Potential Commercial Applications: Competitive Advantages:
  • Investigations into physiological mechanisms of airway diseases, such as cystic fibrosis and asthma
  • Screening of drugs and therapeutic compounds directed to complex, multi-tissue type matrices
  • Biomedical research exploring pharmacology-physiology integration
 
  • Allows simultaneous measurement of transepithelial potential difference, transepithelial resistance, smooth muscle activity and changes in tracheal diameter
  • In vitro analysis of trachea or tracheal segments retaining native, in situ structure
  • Pharmacological agents may be added separately to the lumen for screening purposes
  • First and only such "single-preparation" device allowing for such broad array of data output


Development Stage:
  • Early-stage
  • In vitro data available
  • In situ data available (on-site)
  • Prototype


Inventors:

Jeffrey Fedan (CDC)  ➽ more inventions...

Yi Jing (CDC)  ➽ more inventions...

Michael Van Scott


Intellectual Property:
U.S. Pat: 7,907,999 issued 2011-03-15
US Application No. 60/762,465

Publications:
JIng Y, et. al. PMID 18835555

Licensing Contact:
Karen Surabian, J.D., M.B.A.
Email: karen.surabian@nih.gov
Phone: 301-594-9719

OTT Reference No: E-246-2013/0
Updated: Jan 27, 2014