Laminin A Peptides


This invention relates to peptides and derivatives thereof having laminin-like activity, as well as a pharmaceutical composition of the peptide. The peptides claimed include Serine-Isoleucine-Lysine-Valine-Alanine-Valine (SIKVAV). Methods for promoting increased adhesion and migration of epithelial cells is also disclosed. The peptides have wide usage in research, nerve regeneration and cancer treatment. For example, this invention may be useful as an adhesion and regeneration agent for nerve guides and as an adhesion agent for vascular prosthesis.

Potential Commercial Applications: Competitive Advantages:
  • adhesion agent
  • cancer therapeutic
  • research reagent
 
  • key extracellular matrix component
  • widespread biological activity


Related Invention(s):
E-521-1986-3


Inventors:

Hynda Kleinman (NIDCR)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 5,211,657 issued 1993-05-18
PCT Application No. 07/530,969
US Application No. 08/411,067

Publications:
JJ Barchi, P Russ, B Johnson, A Otaka, M Nomizu, Y Yamada. Glycosylation of the active sequence Ser-Ile-Lys-Val-Ala-Val from (1 chain of laminin reduces tumor cell attachment activity. Bioorganic Medinal Chem Lett 5:711, 1995.
MC Kibbey, B Johnson, R Petryshyn, M Jucker, HK Kleinman. A 110-kD nuclear shuttling protein, nucleolin, binds to the neurite-promoting IKVAV site of laminin-1. J Neurosci Res 1995 Oct 15;42(3):314-22.
RS Bresalier, B Schwartz, YS Kim, QY Duh, HK Kleinman, PM Sullam. The laminin alpha 1 chain Ile-Lys-Val-Ala-Val (IKVAV)-containing peptide promotes liver colonization by human colon cancer cells. Cancer Res 1995 Jun 1;55(11):2476-80.
M Nomizu, BS Weeks, CA Weston, WH Kim, HK Kleinman, Y Yamada. Structure-activity study of a laminin alpha 1 chain active peptide segment Ile-Lys-Val-Ala-Val (IKVAV). FEBS Lett 1995 May 29;365(2-3):227-3.
MC Kibbey, ML Corcoran, LM Wahl, HK Kleinman. Laminin SIKVAV peptide-induced angiogenesis in vivo is potentiated by neutrophils. J Cell Physiol 1994 Jul;160(1):185-93.
BS Weeks, E Holloway, PE Klotman, SK Akiyama, HW Schnaper, HK Kleinman. 12-O-tetradecanoylphorbol 13-acetate stimulates human T-lymphocyte adherence to the fibronectin RGD domain and the laminin IKVAV domain. Cell Immunol 1994 Jan;153(1):94-104.

Licensing Contact:
David Bradley,
Email: bradleyda@nidcr.nih.gov
Phone: 301-402-9242

OTT Reference No: E-246-1988-0
Updated: Oct 1, 1997