Susceptibility-Matched Multiwell Plates for MRI and NMR Spectroscopy


The technology is a patent estate covering multi-well assay plates for high-throughput screening using MRI and Nuclear Magnetic Resonance (NMR) spectroscopy. Conventional multi-well plates typically give poor performance for MRI-based assays since they provide inadequate matching of magnetic susceptibility between the plate, the sample and their surroundings. This results in distortion of the magnetic field within the scanner and reduces the sensitivity for detecting magnetic particles and the resolution of NMR spectra. The plates of this invention are a one-piece polyetherimide plastic construction for improved magnetic susceptibility matching for aqueous samples. Further, susceptibility matching can be achieved by including plugs, individually or as a cap mat, for each well the same plastic as the plate. The plates, with or without plugs, can be filled and manipulated by standard robotic laboratory equipment widely used in the pharmaceutical industry. Multi-well plates are used in high-throughput research applications such as clinical chemistry, immunology, and drug discovery. MR imaging of multi-well plates in particular offers the possibility of detecting magnetic nanoparticles for use as imaging agents. Moreover, MRI-guided localized NMR spectroscopy could be used to perform detailed chemical analysis of complex mixtures of metabolites not possible by conventional analytical techniques. Best of all, conventional MRI techniques exist which permit all samples in one or more multi-well plate(s) to be analyzed simultaneously.

Potential Commercial Applications: Competitive Advantages:
  • NMR spectroscopy
  • MRI imaging of magnetic nanoparticles
  • Clinical chemistry
  • Immunology
  • Drug discovery, combinatorial chemistry, and quality control in the pharmaceutical, chemical and agricultural industries
 
  • Increased signal-to-noise ratio and sensitivity relative to conventional multi-well plates
  • Portability
  • Compatible with existing high-throughput robots


Development Stage:
  • Prototype
  • Pilot
  • Early-stage


Inventors:

Kenneth Fishbein (NIA)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 8,427,160 issued 2013-04-23
US Application No. 12/083,501
PCT Application No. PCT/US2006/039048

Collaboration Opportunity:

The National Institute on Aging, Magnetic Resonance Imaging & Spectroscopy Section, is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize this technology. Please contact Nicole Darack Guyton, Ph.D. at 301-435-3101 or guytonn@mail.nih.gov for more information.


Licensing Contact:
Richard Girards,
Email: richard.girards@nih.gov
Phone: 240-276-5360

OTT Reference No: E-243-2005/0
Updated: Mar 28, 2013