A New Mouse Monoclonal Antibody Against Human Microphthalmia Transcription Factor (MITF)


Micropthalmia Transcription Factor (MITF) plays an important role in melanocyte development and melanoma growth. MITF is important for embryonic development, regulating the generation of pigment cells and formation of melanomas and other tumors. MITF is made in various isoforms that may play unique roles for different organs during different developmental periods. Additionally, tissue MITF levels can serve as a molecular marker for the diagnosis of metastatic melanoma and therapeutic response.

This technology involves the generation of several novel mouse monoclonal antibodies against a sub-domain of an MITF fragment that is cleaved during cell death. Importantly, these antibodies cross-react with human MITF. The antibody was raised by immunizing mice that are incapable of producing the MITF sub-domain used as the antigen. Three (3) different "clones" of these antibodies are currently available and their corresponding hybridoma names are 6A5 (IgG1), 1D2 (IgG2a) and 3D1 (IgG2a).

Potential Commercial Applications: Competitive Advantages:
  • Novel mouse monoclonal antibodies specific to a domain of MITF as research material
  • Novel mouse monoclonal antibodies that cross react with human MITF
 


Development Stage:
The technology is ready for the market.

Inventors:

Heinz Arnheitter (NINDS)  ➽ more inventions...

Wen Fang Liu (NINDS)  ➽ more inventions...

Hideki Murakami (NINDS)  ➽ more inventions...


Intellectual Property:
Research Material -- patent protection is not be pursued for this technology

Publications:
LA Garraway, HR Widlund, MA Rubin, G Getz, AJ Berger, S Ramaswamy, R Beroukhim, DA Milner, SR Granter, J Du, C Lee, SN Wagner, C Li, TR Golub, DL Rimm, ML Meyerson, DE Fisher, WR Sellers. Integrative genomic analyses identify MITF as a lineage survival oncogene amplified in malignant melanoma. Nature 2005 Jul 7;436(7047):117-122. PubMed abs
SR Granter, KN Weilbaecher, C Quigley, DE Fisher. Role for microphthalmia transcription factor in the diagnosis of metastatic malignant melanoma. Appl Immunohistochem Mol Morphol. 2002 Mar; 10(1):47-51. PubMed abs

Licensing Contact:
Susan Ano, Ph.D.
Email: susan.ano@nih.gov
Phone: 301-435-5515

OTT Reference No: E-228-2006/0
Updated: Jun 1, 2006