Influenza A Virus Detection and Subtyping Directly from Animal Swabs and Environmental Samples


Influenza (flu) is a contagious respiratory illness caused by circulating influenza viruses, typically types A and B. The emergence of a new and very different influenza A virus capable of infecting humans may potentially cause an influenza pandemic. To monitor the risk to humans and identify potential sources of infection, CDC scientists have developed a novel method to identify influenza A subtypes in animal and environmental samples using cDNA (clone) hybridization and adapter-mediated amplification. This technology provides a unique strategy for target enrichment, detection, subtyping, real-time quantification, and genetic characterization. The samples are first enriched for influenza A virus through on-bead hybridization. The beads contain degenerative sequences that can bind to a variety of influenza A viruses including Newcastle disease (NCD), which is an influenza-like virus that may be present in Avian samples. The hybridization is performed with a special buffer that enables target enrichment. Thereafter, new influenza A virus subtypes can be detected using real-time PCR with a universal primer. There are no commercial kits currently available that detect the full spectrum of influenza A subtypes.

Potential Commercial Applications: Competitive Advantages:
  • Rapidly and specifically subtype influenza A viruses for diagnostic assays
  • Develop into a kit for veterinary laboratories' sub-typing of animal-origin influenza A viruses
  • Strengthen zoonotic influenza monitoring and surveillance
  • Assist public health laboratories and research institutes for improving prevention and control of influenza
 
  • Detects new influenza A virus subtypes that current commercially available diagnostics cannot
  • Not locked into a particular platform or software - can be combined with sequencing or incorporated into a multiplex assay
  • Helps reduce false positive and false negative results
  • Can detect and quantitatively differentiate up to 5 influenza subtypes within a single reaction tube
  • Straightforward procedure can be automated


Related Invention(s):
E-164-2013/0
E-185-2013/0
E-274-2013/0
E-331-2013/0
E-562-2013/0


Inventors:

Genyan (Patrick) Yang (CDC)  ➽ more inventions...

Charles Davis (CDC)  ➽ more inventions...


Intellectual Property:
US Application No. 62/355,267

Publications:
Yang G, et al. PMID 27372075

Collaboration Opportunity:

The CDC Technology Transfer Office (TTO) is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize: Influenza A Virus Detection and Subtyping Directly from Animal Swabs and Environmental Samples. For collaboration opportunities, please contact CDC TTO at tto@cdc.gov.


Licensing Contact:
Karen Surabian, J.D., M.B.A.
Email: karen.surabian@nih.gov
Phone: 301-594-9719

OTT Reference No: E-199-2016/0
Updated: Feb 27, 2018