Composition and Methods for Rapid Detection of HIV by Loop-mediated Isothermal Amplification


This invention relates to methods and compositions for rapid detection of HIV nucleic acids in a biological sample. Specifically, it involves the use of the loop-mediated isothermal amplification (LAMP) for rapid detection of HIV-1 and/or HIV-2. The use of rapid HIV tests is highly attractive for screening of patient samples, especially in developing countries where resources are limited, because they are quick, easy to perform, and do not require any special equipment. Rapid tests for the identification of HIV antibody, however, will remain negative during the 4 to 5 week window post-infection and pre-seroconversion, necessitating the need for a diagnosis based on HIV nucleic acid.

Potential Commercial Applications: Competitive Advantages:
  • Diagnostic test for HIV-1 and/or HIV-2 infection
  • Kits for detection of HIV nucleic acids
 
  • High sensitivity and specificity.
  • No need for thermal cyclers or gel electrophoresis
  • Assay can be used in limited-resource settings.
  • A rapid, portable and cost-effective alternative to PCR and enzyme immune assays.


Development Stage:
  • Pre-clinical
  • In vitro data available


Inventors:

Kelly Curtis (CDC)  ➽ more inventions...

Donna Rudolph (CDC)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 8,900,807 issued 2014-12-02
PCT Application No. PCT/US2009/035130
US Application No. 12/918,536

Publications:
Curtis KA, et al. PMID 18524393
Curtis KA, et al. PMID 22384022
Curtis KA, et al. PMID 19382260

Licensing Contact:
Jeremiah Mitzelfelt,
Email: jeremiah.mitzelfelt@nih.gov
Phone: 301-443-8518

OTT Reference No: E-173-2013/0
Updated: Nov 20, 2013