Use of Cumulative Distribution Functions to Determine Protein Purity and Homogeneity


Successful solutions to numerous problems in the biochemical sciences depend on the ability to produce "pure" proteins and recognize the degree to which proteins might be modified. Current methods used for assessing purity are relatively nonspecific and insensitive to small differences in molecular weight. The inventors have developed a computer-implemented method and system for nonparametric statistical analysis of matrix-assisted laser desorption ionization (MALDI) protein spectra but is equally applicable to deconvoluted electrospray ionization (ESI) spectra. The invention facilitates assessing protein heterogeneity and detection of otherwise indistinguishable differences in the distribution of molecular weight. A principal advantage is that no additional instrumentation is required beyond that typically included in a mass spectrometry analysis system.

Inventors:

Alfred Yergey (NICHD)  ➽ more inventions...

Paul Blank (NICHD)  ➽ more inventions...

Christin Sjomeling (NICHD)  ➽ more inventions...


Intellectual Property:
US Application No. 09/844,962

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OTT Reference No: E-163-2000/0
Updated: Sep 1, 2000