Multiplex Assay for Rapid Salmonella Serotyping from Human, Animal, Food and Environmental Sources


CDC researchers have developed a bead-based nucleic acid assay for serotyping members of the Salmonella genus; this assay will identify serotypes for approximately 95% of all human-obtained Salmonella isolates in the United States.

Presently, production and quality control for the more than 250 antisera required to cover the >2,500 known serotypes using current methods is difficult, expensive and laborious. Many clinical Salmonella isolates can require three to five days to determine serotype, delaying conclusive serotype identification and postponing alerts to public health monitoring programs. To that end, this new assay provides improved diagnostic identification and discrimination as well as reducing reagent consumption and technician labor. The assay has been developed and optimized in parallel with traditional serotyping methods on a panel of 368 isolates that represents all subspecies/serogroup combinations in the current Kauffmann-White Salmonella classification scheme, and the assay has been shown to be more specific than traditional molecular assay formats. This diagnostic assay will improve the rate and accuracy of Salmonella detection serotyping within human, animal, food and environmental samples.

Potential Commercial Applications: Competitive Advantages:
  • Rapid, simple and accurate Salmonella serotype identification
  • Diagnostic tool for clinical or research settings
  • Food-safety, food-source monitoring
  • Salmonella-outbreak monitoring, prevention and mitigation
 
  • Rapid detection and quantification of Salmonella serotypes from fluidic human, animal, food and environmental samples
  • Increased sensitivity and efficiency compared to current serotyping assays
  • Addresses critical need for improvement in accurate molecular diagnosis for multiple Salmonella serotypes
  • Ready for commercialization, allows for easy addition/modification for future assay expansions
  • Easily implemented as a kit


Development Stage:
  • In vitro data available


Inventors:

Patricia Fields (CDC)  ➽ more inventions...

John McQuiston (CDC)  ➽ more inventions...

Collette Fitzgerald Leaumont (CDC)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 9,115,408 issued 2015-08-25
U.S. Pat: 9,163,287 issued 2015-10-20
US Application No. 61/406,797
US Application No. 13/871,098
US Application No. 13/877,467
PCT Application No. PCT/US2011/057875
PCT Application No. PCT/US2013/038380
Various international patents pending

Publications:
Fitzgerald C, et al. PMID 17634307
McQuiston JR, et al. PMID 21159932

Licensing Contact:
Tara Kirby, Ph.D.
Email: tara.kirby@nih.gov
Phone: 240-669-5128

OTT Reference No: E-149-2013/0
Updated: Jun 27, 2016