Utilization of Non-Viral Sequences for Minus-Strand DNA Transfer and Gene Reconstitution


This technology relates to novel retroviral vectors for the introduction of heterologous nucleic acid into a host cell. Integration of these vectors into the nucleic acid of a host cell results in reconstitution and duplication of the heterologous nucleic acid in the cellular genome. The invention describes a method to efficiently reconstitute genes during virus replication. Vectors have been developed that enable gene reconstitution, by including two halves of a gene, each half having a small region of homology. The 3' half of the gene is inserted into the 5' terminal repeat, before the "R" region, and the 5' half of the gene is inserted into the 3' terminal repeat, between the "U3" region and the "R" region. Upon transfer into a cell and viral integration into the genome, two complete copies of the gene are reconstituted (gene duplication), one in the 5' long terminal repeat (LTR) and one in the 3' LTR. The virus can be used to transfer two copies of genes, such as toxic genes, into a desired cell population, or can be used to detect the presence of competent retroviruses (as a detection system). This technique can be utilized for delivery of toxic genes for cancer gene therapy or for high-sensitivity detection of replication-competent retroviruses during propagation of viral stocks.

Inventors:

Wei-shau Hu (NCI)  ➽ more inventions...

Vinay Pathak (NCI)  ➽ more inventions...


Intellectual Property:
US Application No. 11/208,436
US Application No. 60/205,395
US Application No. PCT/US01/15739

Licensing Contact:
Lauren Nguyen-Antczak, Ph.D., J.D.
Email: lauren.nguyen-antczak@nih.gov
Phone: 301-624-8752

OTT Reference No: E-134-2000-0
Updated: Dec 1, 2000