DNA Probes to Rapidly Screen Candida glabrata Isolates for Echinocandin Resistance to Antifungal Treatment


Candida glabrata is one causative agent of common fungal infections such as candidemia, or Candida (a yeast) present in the bloodstream. Vulnerable populations that can acquire candidemia include immunocompromised individuals such as the elderly and patients receiving chemotherapy. Echinocandins, which disrupt the fungal cell wall, are the most commonly prescribed antifungal treatment for Candida glabrata-induced candidemia. However, mutations in the FKS (FKS1 and FKS2) genes have been shown to cause C. glabrata to become resistant to echinocandins. Currently, resistance to echinocandins is detected by susceptibility testing, which is resource intensive, costly, and requires more than twenty-four hours to process.

CDC scientists have developed and validated a set of DNA probes using the Luminex system that can rapidly detect mutations in C. glabrata FSK genes. Such information could assist physicians in developing an effective treatment strategy for their patients with C. glabrata infection. These DNA probes, used together with Luminex technology or other DNA-based technology such as RT-PCR would allow rapid and high throughput identification of FKS mutations in C. glabrata and provide early detection of resistance.

Potential Commercial Applications: Competitive Advantages:
  • Rapid detection of echinocandin (antifungal) resistance in C. glabrata isolates
  • Medical diagnostic for C. glabrata resistance to echinocandins
  • Monitoring and public health surveillance
 
  • Early detection of echinocandin resistance
  • Rapid and cost-effective
  • Adaptable to low- or high-throughput formats
  • Easy to analyze probe-based assays
  • Allele profiles can be easily determined
  • Newly identified C. glabrata mutations can easily be incorporated into the existing assay


Inventors:

Cau Pham (CDC)  ➽ more inventions...

Shawn Lockhart (CDC)  ➽ more inventions...


Intellectual Property:
US Application No. 62/062,090
US Application No. 14/875,599

Publications:
Pham CD, et al. PMID 24353003

Collaboration Opportunity:

The CDC Technology Transfer Office (TTO) is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize DNA Probes to Rapidly Screen Candida glabrata Isolates for Echinocandin Resistance to Antifungal Treatment. For collaboration opportunities, please contact CDC TTO at tto@cdc.govor1-404-639-1330.


Licensing Contact:
Jeremiah Mitzelfelt,
Email: jeremiah.mitzelfelt@nih.gov
Phone: 301-443-8518

OTT Reference No: E-132-2014/0
Updated: Jan 9, 2018