Nanoparticle Platform Using Bacterial Spore Coat Proteins


Bacterial spores can be modified to display molecules of interest, including drugs, immunogenic peptides, antibodies and other functional proteins of interest (such as enzymes).  The resulting engineered bacterial spores can provide many useful functions such as the treatment of infections, use as an adjuvant for the delivery of vaccines, and the enzymatic degradation of environmental pollutants.

Researchers at the National Cancer Institute’s Laboratory of Molecular Biology have developed a novel, synthetic spore husk-encased lipid bilayer (SSHEL) particle that is uniquely suited for a variety of these functions. The assembly of SSHELs involves the insertion of the bacterial spore coat protein SpoVM into a lipid bilayer that is located on a synthetic core particle.  SpoVM serves as a structural element and recruitment factor for the bacterial spore coat protein SpoIVA.  When SpoIVA is conjugated to streptavidin, the SSHEL can bind to another molecule through a biotin linkage.  This leads to the creation of a specialized SSHEL that can serve a particular biological function.  By varying the ratio of streptavidin-conjugated and unconjugated SpoIVA protein used in the manufacture of the SSHEL, it is possible to tailor the amount of functional molecule that is present in the SSHEL.  This affords greater control over the level of activity that is provided by the SSHEL, thereby allowing the fine tuning of its function.



Potential Commercial Applications: Competitive Advantages:
  • Applications for this technology are only limited by the molecules bound to the nanoparticle (SSHEL)
  • Use of SSHELs that display a therapeutic agent to treat a disease
  • Use of SSHELs to deliver vaccines to patients
  • Use of SSHELs to provide particles with enzymatic functions
 
  • The ability to display multiple types of therapeutic agents provides diversity of function
  • The ability to vary the amount of SpoIVA that can bind to a functional agent (through a streptavidin-biotin interaction) allows fine tuning and greater control of the functional capacity of the SSHEL
  • Lack of extraneous cell surface proteins on SSHELs avoids potential interference between the SSHEL and its target
  • Non-living delivery system avoids complications associated with using a living organism


Development Stage:
Discovery (Lead Identification)

Inventors:

Kumaran Ramamurthi (NCI)  ➽ more inventions...


Intellectual Property:
US Provisional Application No. 62/127,738
U.S. Filed Application No. PCT/US2015/044316

Publications:
Wu et al. A Versatile Nano-Display Platform from Bacterial Spore Coat Proteins PMID: 25854653

Collaboration Opportunity:

Licensing only


Licensing Contact:
John Hewes, Ph.D.
Email: John.Hewes@nih.gov
Phone: 240-276-5515

OTT Reference No: E-098-2015
Updated: Apr 18, 2018