Intracellular Trapping of Radionuclides by Enzyme-Mediated Reduction


The invention provides a novel technique for intracellular trapping of radionuclides for use in cancer therapy and imaging. The technique includes enzyme-mediated intracellular trapping of a radionuclide in a target cell by transfecting the target cell with a transgenic vector encoding a microbial hydrogenase and treating the transfected target cell with a radionuclide. The transgenically expressed microbial hydrogenase catalyzes the reduction of the radionuclide. The reduced radionuclide is trapped intracellularly where its emissions can be detected in radioscintigraphy applications. Emissions from intracellularly trapped radionuclides can also be cytotoxic to the target cell and therefore useful in radiotherapy applications. The invention further provides a reporter mechanism wherein a microbial hydrogenase encoding nucleic acid is included in a vector along with a transgene, both under the control of the same promoter. The detection of emissions from intracellularly reduced and trapped radionuclides is used to monitor transgene expression.

Inventors:

Fangyu Peng (CC)  ➽ more inventions...

King Li (CC)  ➽ more inventions...

Sunil Pandit (CC)  ➽ more inventions...


Intellectual Property:
US Application No. 60/507,607
US Application No. PCT/US2004/031843

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OTT Reference No: E-083-2003/0
Updated: Nov 1, 2003