HPV Virus-Like Particles for Delivery of Gene-Based Vaccines


The invention describes methods of eliciting immune responses and treating disease based on novel vaccine compositions and vaccination strategies employing human papilloma virus (HPV) virus-like particles (VLPs), comprising L1 and L2 proteins. These VLPs have the capacity to incorporate up to 8 kb of DNA into the shell and express only the target antigen. These compositions are effective at eliciting an immune response to the transgene product expressed by the DNA when administered at epithelial surfaces including the mucosa (e.g. nasal or respiratory passages or genital tract) or skin in conjunction with disruption of the epithelial layer. It is typically difficult to elicit an immune response in the genital tract, so this technology overcomes a previous deficiency. Robust B and T cell responses were elicited in mice using the subject technology with representative DNA expressing M/M2 from respiratory syncytial virus (RSV). This technology could be used in a prime-boost vaccination regimen as well to enhance the immune response.

Potential Commercial Applications: Competitive Advantages:
  • Vaccines against a number of pathogens, including HPV, HIV, HSV, HCV, and RSV.
 
  • Novel, non-invasive vaccine strategy to elicit both systemic and mucosal immunity in typically poorly inductive sites
  • Packaging system that can accommodate up to 8 kb of DNA
  • No expression of viral genes
  • Potential for multivalent vaccine development against heterologous pathogens


Development Stage:
Animal (mouse) data available.

Related Invention(s):



Inventors:

Barney Graham (NIAID)  ➽ more inventions...

John Schiller (NCI)  ➽ more inventions...

Christopher Buck (NCI)  ➽ more inventions...

Jeffrey Roberts (NCI)  ➽ more inventions...

Teresa Johnson (NIAID)  ➽ more inventions...

John Nicewonger (NIAID)  ➽ more inventions...

Rhonda Kines (NCI)  ➽ more inventions...


Intellectual Property:
US Application No. 61/022,324
PCT Application No. PCT/US2009/031600
US Application No. 12/863,572

Publications:
Buck CB, et al. Efficient intracellular assembly of papillomaviral vectors. [PMID 14694107]

Licensing Contact:
Kevin Chang, Ph.D.
Email: changke@mail.nih.gov
Phone: 240-276-6910

OTT Reference No: E-077-2008/0
Updated: Mar 14, 2018