Detection and Discrimination of Classical and Atypical L-Type BSE Strains by RT-QuIC


Statutory surveillance of bovine spongiform encephalopathy (BSE) indicates that cattle are susceptible to both classical (C-BSE) and atypical forms of BSE. Atypical forms of BSE appear to be sporadic and thus may never be eradicated. A major challenge is the lack of sufficiently practical and sensitive tests for routine BSE detection and strain discrimination. The RT-QuIC test, which is based on prion-seeded fibrillization of recombinant prion protein (rPrPSen), is known to be highly specific and sensitive for detection of multiple human and animal prion diseases, but not BSE. This application claims methods for distinguishing whether a sheep, cow or goat has atypical L-bovine spongiform encephalopathy prion or classical bovine spongiform encephalopathy.

Potential Commercial Applications: Competitive Advantages:
  • Detection and distinguishing of both BSE forms
  • Rapid detection and discrimination of BSE forms
 
  • Orders of magnitude more sensitive than ELISA tests
  • Eliminates need for multi-phase analyses of samples
  • Can be applied to large scale testing of multiple samples


Development Stage:
  • In vitro data available
  • In vivo data available (animal)
  • Prototype


Inventors:

Byron Caughey (NIAID)  ➽ more inventions...

Christina Groveman (NIAID)  ➽ more inventions...

Alessandra Favolez

Cristina Casalone

Maria Mazza

Cristiano Corona


Intellectual Property:
US Application No. 62/092,645

Publications:
Orru CD, et al. PMID 25609728
Orru CD, et al. PMID 26284358
Orru CD, et al. PMID 26086786

Licensing Contact:
Jeffrey Thruston,
Email: jeffrey.thruston@nih.gov
Phone: 301-594-5179

OTT Reference No: E-048-2015-0
Updated: May 7, 2018