Methods for Preparing Immunogenic Conjugates


This technology describes improved methods of synthesis for conjugate vaccines, specifically those against anthrax. The inventors' method is designed to synthesize immunogenic conjugates (i.e., a protein carrier conjugated to a bacterially derived synthetic peptide) that are prepared at a physiological pH, not reversible and do not require reduction with borohydride. The inventors' method comprises reacting the protein carrier with a dihydrazide, and the peptide with a benzaldehyde, or the reverse, then reacting the derivatized peptide and the derivatized protein with each other to form an immunogenic conjugate.

Potential Commercial Applications: Competitive Advantages:
  • Methods for making conjugate vaccines and reagents.
 
  • More efficient conjugation methods, higher conjugate yields.


Development Stage:
Vaccine candidates have been synthesized and preclinical studies have been performed.

Inventors:

Rachel Schneerson (NICHD)  ➽ more inventions...

Joanna Kubler-kielb (NICHD)  ➽ more inventions...

Fathy Majadly (NICHD)  ➽ more inventions...

Stephen Leppla (NIAID)  ➽ more inventions...

John Robbins (NICHD)  ➽ more inventions...

Darrell Liu (NICHD)  ➽ more inventions...

Joseph Shiloach (NIDDK)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 7,625,736 issued 2009-12-01
U.S. Pat: 8,481,048 issued 2013-07-09
US Application No. 12/582,420
International rights available

Publications:
Kubler-Kielb J, et al. PMID 16861662

Licensing Contact:
Martha Lubet, Ph.D.
Email: lubetm@mail.nih.gov
Phone: 240-276-5508

OTT Reference No: E-040-2005-0
Updated: May 4, 2018