Method For Immune Capture And Primary Isolation Of Borrelia Burgdorferi


This invention relates to novel antigens associated with Borrelia burgdorferi which are exported (or shed) in vivo and whose detection is a means of diagnosing Lyme disease. The antigens are extracellular membrane vesicles and other bioproducts including the major extracellular protein. The invention further provides antibodies, monoclonal and/or polyclonal, labeled and/or unlabeled, that react with the antigens. The invention is also directed to a method of diagnosing Lyme disease by detecting the antigens in a biological sample taken from a host using the antibodies in conventional immunoassay formats. The invention further relates to kits, for the diagnosis of Lyme disease, comprising the antibodies and ancillary reagents. The advantage of the antibodies used in the invention is that they react with the antigens from geographically diverse strains of Borrelia burgdorferi, but do not react with antigens from related Borrelia spirochetes.

Inventors:

David Dorward (NIAID)  ➽ more inventions...

Tom Schwan (NIAID)  ➽ more inventions...

Claude Garon (NIAID)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 5,403,718 issued 1995-04-04

Licensing Contact:
Benjamin Hurley, Ph.D.
Email: benjamin.hurley@nih.gov
Phone: 240-669-5092

OTT Reference No: E-032-1990/1
Updated: Feb 1, 1994