TRRAP and GRIN2A Mutations for the Diagnosis and Treatment of Melanoma


Using whole-exome sequencing of matched normal and metastatic tumor DNAs, researchers at the NIH have identified several novel somatic (e.g., tumor-specific) alterations, many of which have not previously been known to be genetically altered in tumors or linked to melanoma. In particular, the researchers identified a recurrent “hotspot” mutation in the transformation/transcription domain-associated protein (TRRAP) gene, found the glutamate receptor ionotropic N-methyl D-aspartate 2A (GRIN2A) gene as a highly mutated in melanoma, and have shown that the majority of melanoma tumors have alterations in genes encoding members of the glutamate signaling pathway. Therefore, this technology not only provides a comprehensive map of genetic alterations in melanoma, but has important diagnostic and therapeutic applications. Mutations in the TRRAP and GRIN2A genes can be used as diagnostic markers for melanoma and may serve as therapeutic targets in the treatment of melanoma. In addition, glutamate antagonists have previously been shown to inhibit proliferation of human tumor cells, and therefore further investigation of the pathway in melanoma could allow for the identification of new therapeutic proteins that target this pathway.

Potential Commercial Applications: Competitive Advantages:
  • Diagnostic array for the detection of TRRAP and GRIN2A mutations.
  • Method of identifying TRRAP and GRIN2A inhibitors as therapeutic agents to treat malignant melanoma patients.
  • Method of selecting a therapy based on the presence of TRRAP and GRIN2A mutations.
 
  • Complete analysis of melanoma exome alterations.
  • TRRAP, GRIN2A, and the other identified mutations are highly frequent and/or highly mutated in melanomas.
  • Glutamate antagonists have already been shown to inhibit tumor growth. Thus, this technology may prove useful for the development of novel diagnostic tests and therapeutics.


Development Stage:
Pre-clinical

Related Invention(s):
E-272-2008-0


Inventors:

Yardena Samuels (NHGRI)  ➽ more inventions...

Xiaomu Wei (NHGRI)  ➽ more inventions...


Intellectual Property:
U.S. Pat: 9,157,125 issued 2015-10-13
PCT Application No. PCT/US2012/022687
US Application No. 13/982,392
Research Tool — Patent protection is not being pursued for the TRRAP and GRIN2A melanoma metastatic cell lines.

Publications:
Wei X, et al. PMID 21499247

Licensing Contact:
Eggerton Campbell, Ph.D.
Email: eggerton.campbell@nih.gov
Phone: 301-402-1648

OTT Reference No: E-013-2011-0
Updated: May 8, 2018