Tissue Microarray For Rapid Molecular Profiling


Recent advances in molecular medicine have provided new opportunities to understand cellular and molecular mechanisms of disease and to select appropriate treatment regimens with the greatest likelihood of success. The clinical application of novel molecular, genetic and genomic discoveries has been impeded by the slow and tedious process of evaluating biomarkers in large numbers of clinical specimens. The present invention provides a method of high-throughput molecular profiling of very large numbers of tissue specimens, such as tumors, with minimal tissue requirements. This procedure provides a target for rapid parallel analysis of biological and molecular characteristics (such as gene dosage and expression) from hundreds of morphologically controlled tumor specimens. Multiple sections can be obtained from such tissue microarrays ("tissue chips") so that each section contains hundreds or thousands of different tissue specimens that maintain their assigned locations. Different in situ analyses, such as histological, immunological, or molecular, are performed on each section to determine the frequency and significance of multiple molecular markers in a given set of tissues. This method can also be combined with other technologies such as high-throughput genomics surveys using DNA microarrays. DNA microarrays enable analysis of thousands of genes from one tissue specimen in a single experiment, whereas the tissue microarrays make it possible to analyze hundreds or thousands of tissue specimens in a single experiment using a single gene or protein probe. Together the DNA and tissue microarray technologies will be very powerful for the rapid analysis of markers associated with disease prognosis or therapy outcome.

Inventors:

Olli Kallioniemi (NHGRI)  ➽ more inventions...

Juha Kononen (NHGRI)  ➽ more inventions...

Guido Sauter

Intellectual Property:
US Application No. 60/106,038

Licensing Contact:
Eggerton Campbell, Ph.D.
Email: eggerton.campbell@nih.gov
Phone: 301-402-1648

OTT Reference No: E-007-1999/0
Updated: Jan 1, 1999