Technology ID
E-276-2013-0

Multiplex Real-time PCR Assay for Detection of Numerous Bacterial Pathogens

Linked ID
TAB-2645
Inventors
Bernard Wolff (CDC)
Jonas Winchell (CDC)
Maureen Diaz (CDC)
Lead Inventors
Jonas Winchell (CDC)
Co-Inventors
Bernard Wolff (CDC)
Maureen Diaz (CDC)
Development Status
  • Pre-clinical
  • In vitro data available
Applications
Diagnostics
Therapeutic Areas
Infectious Disease
ICs
CDC
Commercial Applications
  • Clinical diagnostic for several pathogens
  • Drug-resistance surveillance
  • Public health monitoring
  • En masse food-safety screening
In order to address a global need for rapid, cost-effective, sensitive, and specific assays for many pathogens, CDC scientists have developed a broad-use, multiplexed RT-PCR assay. This comprehensive assay covers numerous pathogens that are common causes of infection in neonates and also important to food-safety. Specifically, this assay (and respective probes, primers, and kits) is capable of detecting one or more of Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Toxoplasma gondii, Moraxella catarrhalis, Escherichia coli, Shigella, Staphylococcus aureus, Pneumocystis jirovecii, Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Ureaplasma spp., Bartonella spp., Streptococcus agalactiae, and Neisseria meningitidis in a biological sample.
Competitive Advantages
  • Cost-effective
  • Simple to implement
  • Rapid, accurate and objectively conclusive
  • Easily implemented into kit format
  • Ideal for high-throughput scenarios

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