Technology ID

One-step random amplification method to detect extremely low input nucleic acids for virome, microbiome, and metagenomics in clinical and biological specimens

Linked ID
Fei Fan (Terry) Ng (CDC)
Lead Inventors
Fei Fan (Terry) Ng (CDC)
Development Stages
Pre-Clinical (in vitro)
Commercial Applications
  • Virome, microbiome, and metagenomics research using direct, non-culturable samples from humans, animals, and the environment
  • Amplification to detect viruses and microbes in clinical specimens – especially when input nucleic acid is low or when rapid, random amplification is needed
  • Agnostic amplification, or testing sample without prior sequence knowledge
  • One-step whole genome or whole transcriptome amplification kits
  • Amplification of clinical samples in the field, such as remote areas or during an outbreak
  • Monitoring and public health surveillance research
Competitive Advantages
  • Rapid one-step or two-step protocol with few hands-on procedures with high sensitivity
  • Can amplify either DNA or RNA from biological samples
  • Offers nonbiased amplification of all nucleic acids in a sample
  • Allows high-throughput processing of samples, including both culturable and non-culturable samples, and low to high yield nucleic acids
  • Provides a broadly applicable method when using universal primers and eliminates the need for microorganism-specific primer design

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