Technology ID
E-161-2017-0

One-step random amplification method to detect extremely low input nucleic acids for virome, microbiome, and metagenomics in clinical and biological specimens

Linked ID
TAB-3371
Inventors
Fei Fan (Terry) Ng (CDC)
Lead Inventors
Fei Fan (Terry) Ng (CDC)
Development Stages
Pre-Clinical (in vitro)
ICs
CDC
Commercial Applications
  • Virome, microbiome, and metagenomics research using direct, non-culturable samples from humans, animals, and the environment
  • Amplification to detect viruses and microbes in clinical specimens – especially when input nucleic acid is low or when rapid, random amplification is needed
  • Agnostic amplification, or testing sample without prior sequence knowledge
  • One-step whole genome or whole transcriptome amplification kits
  • Amplification of clinical samples in the field, such as remote areas or during an outbreak
  • Monitoring and public health surveillance research
Competitive Advantages
  • Rapid one-step or two-step protocol with few hands-on procedures with high sensitivity
  • Can amplify either DNA or RNA from biological samples
  • Offers nonbiased amplification of all nucleic acids in a sample
  • Allows high-throughput processing of samples, including both culturable and non-culturable samples, and low to high yield nucleic acids
  • Provides a broadly applicable method when using universal primers and eliminates the need for microorganism-specific primer design

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