Technology ID
E-145-2013-0

Real-Time RT-PCR Assay for Detection of Noroviruses

Linked ID
TAB-2600
Inventors
Jan Vinje (CDC)
Leslie Barclay (CDC)
Nicole Gregoricus (CDC)
Preeti Chhabra (CDC)
Lead Inventors
Jan Vinje (CDC)
Co-Inventors
Leslie Barclay (CDC)
Nicole Gregoricus (CDC)
Preeti Chhabra (CDC)
Development Status
  • Pre-clinical
  • In vitro data available
Applications
Diagnostics
Therapeutic Areas
Infectious Disease
ICs
CDC
Commercial Applications
  • Development of norovirus diagnostics
  • Specific rtRT-PCR assay for detecting and distinguishing of the major pathogenic norovirus genogroups (I and II) within clinical and environmental samples
A specific and sensitive TaqMan-based real-time (rt) RT-PCR assay has been developed by CDC scientists for detection of noroviruses in clinical and environmental specimens. This assay can be implemented to rapidly detect and distinguish norovirus strains from genogroups I and II, which are responsible for the majority of human infections. Additionally, the assay is multiplexed with an internal extraction control virus (coliphage MS2) to validate the results of the assay. Since the virus cannot be grown in cell culture and enzyme immunoassays lack the necessary sensitivity, this technology is particularly useful.
Competitive Advantages
  • This is an internally controlled, multiplexed assay capable of rapid, accurate identification of norovirus genogroups responsible for human illness
  • Superior sensitivity compared with immunoassay detection methods

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