Technology ID
E-108-2013-0

Chicken Polyclonal Antiserum to the Nitrone Spin Trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO)

Linked ID
TAB-2957
Inventors
Marilyn Ehrenshaft (NIEHS)
Ronald Mason (NIEHS)
Lead Inventors
Ronald Mason (NIEHS)
Co-Inventors
Marilyn Ehrenshaft (NIEHS)
Applications
Research Materials
ICs
NIEHS
Commercial Applications
  • The chicken polyclonal anti-DMPO can be used to detect the stable nitrone end-product of protein and DNA radicals in ELISA assays, blot analyses and confocal microscopy.
8/03/2015 -- abstract below supplied by the IC for posting at the OTT website TAB-2957 (http://www.ott.nih.gov/technology/e-108-2013) Chicken Polyclonal Antiserum to the Nitrone Spin Trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) Biological radicals resulted from oxidative stress has been implicated in human diseases, such as cancer and aging. There is, however, a paucity of reliable methods for in vivo or ex vivo detection of either radical formation, the end-products of radical formation or susceptibility for radical formation. The chicken polyclonal anti-DMPO can be used to detect the stable nitrone end-product of protein and DNA radicals in ELISA assays, blot analyses and confocal microscopy. This chicken polyclonal anti-DMPO antiserum will supplement both the mouse monoclonal anti-DMPO and the rabbit polyclonal anti-DMPO. The availability of anti-DMPO from three different sources will greatly expand the ability of researchers to use anti-DMPO in conjunction with other antibodies. Application: The chicken polyclonal anti-DMPO can be used to detect the stable nitrone end-product of protein and DNA radicals in ELISA assays, blot analyses and confocal microscopy. Advantages: DMPO is one of the most frequently used spin traps to detect free radicals and has been cited in over 1000 articles (Pubmed). The availability of anti-DMPO of different origins (rabbit, mouse, chicken) will increase the ability of the researcher to use anti-DMPO in conjuction with other antibodies of interest.
Competitive Advantages
  • DMPO is one of the most frequently used spin traps to detect free radicals and has been cited in over 1000 articles (PubMed). The availability of anti-DMPO of different origins (rabbit, mouse, chicken) will increase the ability of the researcher to use anti-DMPO in conjuction with other antibodies of interest.

Request More Info

Licensing Contact