Marilyn Ehrenshaft (NIEHS)
Biological radicals resulted from oxidative stress has been implicated in human diseases, such as cancer and aging. There is, however, a paucity of reliable methods for in vivo or ex vivo detection of either radical formation, the end-products of radical formation or susceptibility for radical formation. The chicken polyclonal anti-DMPO can be used to detect the stable nitrone end-product of protein and DNA radicals in ELISA assays, blot analyses and confocal microscopy. This chicken polyclonal anti-DMPO antiserum will supplement both the mouse monoclonal anti-DMPO and the rabbit polyclonal anti-DMPO. The availability of anti-DMPO from three different sources will greatly expand the ability of researchers to use anti-DMPO in conjunction with other antibodies.
- The chicken polyclonal anti-DMPO can be used to detect the stable nitrone end-product of protein and DNA radicals in ELISA assays, blot analyses and confocal microscopy.
- DMPO is one of the most frequently used spin traps to detect free radicals and has been cited in over 1000 articles (PubMed). The availability of anti-DMPO of different origins (rabbit, mouse, chicken) will increase the ability of the researcher to use anti-DMPO in conjuction with other antibodies of interest.