Technology Bundle ID: TAB-2936

Rapid Method for the Detection of Antigen-Specific Antibodies in Any Species

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Primary Inventors: 
Alison Basile (CDC)
Development Stage: 
Pre-Clinical (in vitro)
Development Status: 

In vitro data available

Institute or Center: 
CDC

Currently available identification methods for antigen-specific antibodies require live pathogens, antisera (that are only available for a limited number of species), and species-specific secondary antibodies (also a limited resource). Thus, detection or surveillance of pathogens in wild avian species or zoo animals, for example, is complex and cumbersome.

Researchers at the CDC have developed methods to rapidly detect antigen-specific antibodies in any species, including those for which secondary antibodies and/or antisera are unavailable. The unique methods involve direct labelling of biological samples, such as with biotinylation, thereby eliminating the need for species-specific conjugates. Thereafter, target-antigen detection can be completed by using a flow instrument or a plate-based immunological assay. Also, the assay can screen a large number of samples and can be adapted for use with many etiologic agents relevant to the mammalian, reptilian, and avian species, plants, or insects. Furthermore, this assay can detect one or more antibody classes simultaneously, rather than being limited to one particular type.

Applications:
  • Pathogen detection/diagnostic for veterinary applications, especially useful in wild or exotic species.
  • Research tool for surveillance studies of pathogens in any species.
Advantages:
  • Universal antigen-specific antibody detection in any species.
  • Rapid and safe screen that can detect one or more antibody classes simultaneously.
  • The assay is not limited by species-specific secondary antibody or antisera availability.
  • The assay can screen a variety of samples, such as serum, plasma, blood, urine, feces, saliva, tears, semen, mucous, or cerebral spinal fluid.

Patents

PCT Application PCT/US2009/054916
Filed on 2009-08-25
US Application 61/093,605
Filed on 2008-09-02
US Pat 9,063,150

Issued 2015-06-23

Foreign counterparts pending in Canada, Europe, and Australia

Publications

Blitvich BJ, et al.
PMID 12791902
Johnson AJ, et al.
PMID 15879016

Updated

May 19, 2015

Data Source: 
tts