Technology Bundle ID: TAB-2820

Discrete Cross-Reactive Epitopes for Flavivirus Serological Diagnostics and Vaccines

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Licensing Contact:
Primary Inventors: 
Gwong-Jen Chang (CDC)
Co-Inventors: 
Wayne Crill (CDC)
Development Stage: 
Pre-Clinical (in vitro)
Development Status: 
  • In vitro data available
Institute or Center: 
CDC

CDC researchers have identified and characterized discrete flavivirus cross-reactive epitopes useful for improving serodiagnosis and vaccination of flaviviruses, such as dengue virus, yellow fever virus, Japanese encephalitis virus, West Nile virus and the tick-borne encephalitis viruses.

The flavivirus envelope glycoprotein is one of the primary antigens inducing protective immunity in a host. Human flavivirus infections stimulate virus species-specific as well as flavivirus-genus cross-reactive immune responses. These cross-reactive antibodies create a serious problem for serodiagnosis, especially for secondary flavivirus infections, due to the difficulty of differentiating primary from secondary cross-reactive serum antibodies. Additionally, the presence of subneutralizing levels of flavivirus cross-reactive serum antibodies in an individual may result in a dramatic increase in the severity of secondary flavivirus infections via antibody-dependent enhancement (ADE), as commonly seen with multi-serotype dengue virus infection.

To that end, the identification and characterization of amino acids incorporated into these flavivirus cross-reactive epitopes extends practical understanding of structure-function relationships important for flavivirus immunopathology. This CDC antigen-epitope technology provides important tools and insights for improving flavivirus serodiagnosis, researching the pathogenesis of ADE, and advancing the development of safe and effective flavivirus vaccines.

Applications:
  • Flavivirus vaccine development
  • Improved serological diagnostics for flaviviruses such as dengue, yellow fever, Japanese encephalitis, West Nile and the tick-borne encephalitis viruses
  • Research of flavivirus-related antibody-dependent enhancement (ADE) pathologies
Advantages:
  • Technology circumvents flavivirus serodiagnostic issues associated with cross-reactive antibody detection and non-specific flavivirus infection diagnoses
  • May provide unique solutions for development of flavivirus-genus or species-specific vaccines

Patents

PCT Application PCT/US2005/026672
Filed on 2005-07-27
US Application 60/591,898
Filed on 2004-07-27
US Pat 7,906,292

Issued 2011-03-15
US Pat 9,000,141

Issued 2015-04-07
US Pat 9,650,422

Issued 2017-05-16

Various international patents pending and issued

Publications

Chang GJ, et al.
PMID 12620809
Crill WD, et al.
PMID 15564505

Updated

May 15, 2014

Data Source: 
tts