Technology Bundle ID: TAB-2741

Fluorescent Primer(s) Creation for Nucleic Acid Detection and Amplification

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Licensing Contact:
Primary Inventors: 
Jothikumar Narayanan (CDC)
Co-Inventors: 
Vincent Hill (CDC)
Development Stage: 
Pre-Clinical (in vitro)
Development Status: 

In vitro data available

Institute or Center: 
CDC

CDC researchers have developed technology that consists of a simple and inexpensive technique for creating fluorescent labeled primers for nucleic acid amplification. Fluorescent chemical-labeled probes and primers are extensively used in clinical and research laboratories for rapid, real-time detection and identification of microbes and genetic sequences. During nucleic acid amplification, the "UniFluor" primer is incorporated into newly synthesized double stranded DNA. As a consequence, quenching of the dye's fluorescent signal occurs decreasing the fluorescence of the sample several fold. The decrease in fluorescence can be measured and observed using any commercially available nucleic acid amplification system that measures fluorescence (e.g., real-time PCR/thermocyclers). Because many real-time PCR applications require a multitude of fluorescently labeled primers or probes, the single-labeled primer technique also allows researchers and clinicians to perform their work at lower cost.

Applications:
  • Quantitative detection and/or amplification of specified nucleic acid sequences
  • Efficient fluorescence-labeling of oligonucleotides
  • Pyro-sequencing
  • Basic laboratory research
Advantages:
  • Simple to implement
  • Rapid, real-time detection
  • Used with standard laboratory equipment capable of monitoring fluorescence-intensity shifts
  • Cost-effective
  • Easily adapted for use in kits or arrays

Patents

PCT Application PCT/US2006/000175
Filed on 2006-01-03
US Application 11/325,270
Filed on 2006-01-03
US Application 60/641,303
Filed on 2005-01-03
US Pat 7,709,626

Issued 2010-05-04

Several international patent applications pending or issued

Updated

Mar 9, 2020

Data Source: 
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