Technology Bundle ID: TAB-2738

Detection of Retroviruses and HIV-1 Groups -M and -O Discrimination within Clinical Serum Samples

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Primary Inventors: 
Walid Heneine (CDC)
Shinji Yamamoto (CDC), Thomas Folks (CDC), William Switzer (CDC)
Development Stage: 
Pre-Clinical (in vitro)
Development Status: 

In vitro data available

Institute or Center: 

CDC researchers have developed methods for detecting retroviruses within a patient blood sample and discriminating HIV-1 samples within serum specimens. HIV-1 can be genetically classified into two major groups, group M (major) and Group O (outlier) with group O comprising all divergent viruses that do not cluster with group M. The identification of group O infections raised public health concerns about the safety of the blood supply because HIV-1 screening by group M-based serologic tests does not consistently detect group O infection.

The assay is based on the selective inhibition of Amp-RT reactivity of Group M viruses by nevirapine, a non-nucleoside RT inhibitor. Group O viruses can be generically identified by the resistance of their Amp-RT activity to nevirapine. The assay can be used to screening of the blood supply and to rapidly differentiate group M from group O virus.

  • Clinical monitoring of individual patient antiretroviral therapy
  • HIV/AIDS public health programs
  • Surveillance of retroviral drug resistance
  • Screening of blood donations
  • Rapid diagnostic which greatly reduces time and labor for improved clinical monitoring of HIV treatment
  • Ready for commercialization
  • Easily adapted to kit format
  • Assists continued usefulness of common antiretroviral therapeutics
  • Useful for high-throughput serum samples screening


PCT Application PCT/US1996/001257
Filed on 1996-01-26
US Application 08/379,851
Filed on 1995-01-27
US Pat 5,849,494

Issued 1998-12-15
US Pat 6,136,534

Issued 2000-10-24

Various international patents issued

Various international patents issued or pending


Heneine W, et al.
PMID 7538549
Reisler RB, et al.
PMID 11176273
Yamamoto S, et al.
PMID 8882946


Jan 27, 2014

Data Source: