Technology Bundle ID: TAB-2724

Method for Finding Usable Portion of Sigmoid Curve (the Taylor Method), Improved Assay Readouts, and Enhanced Quality Control/Assurance

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Primary Inventors: 
Thomas Taylor (CDC)
Development Stage: 
Pre-Clinical (in vitro)
Development Status: 

In vitro data available

Institute or Center: 
CDC

CDC researchers have developed algorithmic methods for determining sigmoid curve optimums and calculating component concentrations. Sigmoid curves are commonly generated in bioassays and used to calculate results. Various techniques have been used to define the curve, analyze the observations, and calculate a concentration. This technology is an algorithmic approach to identifying the usable portion of a sigmoid curve. This approach is more objective than other methods, reducing the variability introduced by individuals and/or by repetition and allows substantially higher throughput in a situation where a lot of samples are being analyzed using the same assay.

Applications:
  • Observation and data analysis
  • Determining concentrations
  • Improving calculations and estimations
  • Enhancing consistency and reproducibility of outcomes for bio and chem assays
Advantages:
  • Less output-data subjectivity than alternate methods
  • Rapid, accurate and simple to implement
  • Quality control and assurance for a number of assays such as PCR, ELISA, toxin neutralization assays (TNA), flow cytometry, cell death assays, titrations, etc.
  • Reduces data variability due to errant input
  • Easily adapted to high-throughput analyses
  • Demonstrated efficacy quantifying anthrax lethal toxin neutralization activity

Patents

PCT Application PCT/US2004/008566
Filed on 2004-03-19
US Application 60/456,613
Filed on 2003-03-21
US Pat 7,469,186

Issued 2008-12-23

Various international patent applications pending or issued

Publications

Li H, et al.
PMID 18304568

Updated

Jan 16, 2014

Data Source: 
tts