Technology Bundle ID: TAB-2722

Universal Diagnostic Assay for Detection and Identification of Poxviruses in Clinical Samples

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Licensing Contact:
Primary Inventors: 
Yu Li (CDC)
Co-Inventors: 
Hui Zhao (CDC), Inger Damon (CDC)
Development Stage: 
Pre-Clinical (in vitro)
Development Status: 

In vitro data available

Institute or Center: 
CDC

CDC researchers have developed an assay for detection and diagnosis of poxviruses within clinical samples or from lab culture-systems. The assay specifically targets chordopoxviruses (except avipoxviruses) for PCR-based identification; an improvement upon the current standard of cell culturing methodologies. Individual chordopoxvirus species can cause disease in humans (e.g., vaccinia, cowpox, monkeypox/Molluscum contagiosum) and animals (e.g., sheeppox, myxoma, swinepox, mule deer pox, tanapox/Orf virus, Bovine popular stomatitis virus). Some poxvirus species impart unique and obvious symptoms making them easy to diagnose, while many others are clinically ambiguous. For instance, parapoxvirus infections are often misdiagnosed as cutaneous anthrax, which unnecessarily contributes to overuse of antibacterial agents. There is therefore a demonstrated need to develop better diagnostic tools to detect and properly identify the agent of poxvirus infections. Regardless of the symptoms, this universal assay can quickly and reliably detect chordopoxvirus presence in clinical samples, allowing for proper identification, diagnosis, treatment, and improved patient outcomes.

Applications:
  • Nucleic acid-based diagnostic for 'unknown rash' illnesses and identifying novel poxviruses
  • Disease surveillance programs, including public health and veterinary (livestock, domestic, wild/exotic)
Advantages:
  • Rapid and simple
  • Allows for high-throughput, simultaneous sample screening
  • Detects, identifies all low-G/C content non-avipox chordopoxviruses and most known high-G/C content chordopoxviruses

Patents

PCT Application PCT/US2010/055061
Filed on 2010-11-02
US Application 61/257,582
Filed on 2009-11-03
US Pat 8,859,742

Issued 2014-10-14

Various international patent applications pending

Publications

Li Y, et al.
PMID 19906902

Updated

Jan 16, 2014

Data Source: 
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