Technology Bundle ID: TAB-2641

Real-time PCR Assay for Detection of Pneumococcal DNA and Diagnosis of Pneumococcal Disease

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Licensing Contact:
Primary Inventors: 
Maria Da Gloria Carvalho (CDC)
Co-Inventors: 
Edwin Ades (CDC), George Carlone (CDC), Jacquelyn Sampson (CDC), Karen McCaustland (CDC)
Therapeutic Area: 
Infectious Disease
Application: 
Diagnostics
Development Status: 
  • Pre-clinical
  • In vitro data available
Institute or Center: 
CDC

CDC scientists have developed a real-time PCR assay for diagnosing pneumococcal disease using amplification of the bacterial gene encoding pneumococcal surface adhesin A (PsaA). Pneumococcal isolation and identification is often complicated by 1) antimicrobial suppression of growth in culture and 2) contamination by normal flora alpha-streptococci. Further, pneumococcal detection by culture and serological methods can be time-consuming, relatively expensive, laborious and, ultimately, indeterminate. Sensitive and specific assays that can be completed quickly in the clinical laboratory are essential for early diagnosis and effective therapy. This RT-PCR assay provides a tool for quick and accurate diagnosis by physicians and health care technicians and may be useful in evaluating the efficacy of novel pneumococcal vaccines and therapeutics.

Applications:
  • Pneumococcal disease diagnostics and surveillance programs
  • Streptococcus pneumoniae vaccine development and improvementt
  • Evaluation of efficacy of anti-pneumococcal therapeutics
Advantages:
  • Cost-effective
  • Simple to implement
  • Rapid, accurate and objectively conclusive
  • Easily implemented as a kit

Patents

PCT Application PCT/US2005/010449
Filed on 2005-03-28
US Pat 7,476,733

Issued 2009-01-13

Publications

Carvalho MG, et al.
PMID 17537936

Updated

Sep 17, 2013

Data Source: 
tts