Real-time PCR Multiplex Assay for Detection of Bacterial Respiratory Pathogens in Clinical Specimens

CDC researchers have developed a single-tube, real-time PCR assay for the simultaneous detection of three bacterial respiratory pathogens (Mycoplasma pneumoniae, Chlamydiophila pneumoniae and Legionella spp.). The assay has an internal control testing for presence of human DNA. This four-plex real-time PCR assay could potentially become a routine screening test for patients with respiratory illness. Ninety four clinical specimens (in a 96-well format) can be tested at once. This assay is non-invasive, rapid and cost-effective. It has the potential for point-of-care applications in population-based pneumonia surveillance.

Potential Commercial Applications: Competitive Advantages:
  • Population-based pneumonia surveillance
  • Development of broadly-capable respiratory clinical diagnostics
  • Sensitive and specific
  • High-throughput friendly
  • Rapid and cost-effective compared to screening for individual respiratory pathogens
  • Easily developed for use in diagnostic kits

Jonas Winchell (CDC)
Agnes Warner (CDC)
Kathleen Thurman (CDC)

Intellectual Property:
PCT Application No. PCT/US2011/032749
US Application No. 61/324,986
US Application No. 13/641,444

Licensing Contact:
Tara Kirby, Ph.D.
NIH Office of Technology Transfer
Phone: 301-435-4426

OTT Reference No: E-300-2013/0

Updated: Sep-19-2013