Multiplex Real-time PCR Assay for Detection of Numerous Bacterial Pathogens


In order to address a global need for rapid, cost-effective, sensitive, and specific assays for many pathogens, CDC scientists have developed a broad-use, multiplexed RT-PCR assay. This comprehensive assay covers numerous pathogens that are common causes of infection in neonates and also important to food-safety. Specifically, this assay (and respective probes, primers, and kits) is capable of detecting one or more of Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Toxoplasma gondii, Moraxella catarrhalis, Escherichia coli, Shigella, Staphylococcus aureus, Pneumocystis jirovecii, Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Ureaplasma spp., Bartonella spp., Streptococcus agalactiae, and Neisseria meningitidis in a biological sample.

Potential Commercial Applications: Competitive Advantages:
  • Clinical diagnostic for several pathogens
  • Drug-resistance surveillance
  • Public health monitoring
  • En masse food-safety screening
 
  • Cost-effective
  • Simple to implement
  • Rapid, accurate and objectively conclusive
  • Easily implemented into kit format
  • Ideal for high-throughput scenarios


Inventors:
Jonas Winchell (CDC)
Bernard Wolff (CDC)
Maureen Diaz (CDC)


Intellectual Property:
US Application No. 61/642,091
PCT Application No. PCT/US2013/028034


Licensing Contact:
Whitney Blair ,
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325
Rockville , MD 20852
Email: blairw2@mail.nih.gov
Phone: 301-435-4937
Fax: 301-402-0220

OTT Reference No: E-276-2013/0

Updated: 09/18/2013