Real-Time RT-PCR Assay for Detection of Noroviruses


A specific and sensitive TaqMan-based real-time (rt) RT-PCR assay has been developed by CDC scientists for detection of noroviruses in clinical and environmental specimens. This assay can be implemented to rapidly detect and distinguish norovirus strains from genogroups I and II, which are responsible for the majority of human infections. Additionally, the assay is multiplexed with an internal extraction control virus (coliphage MS2) to validate the results of the assay. Since the virus cannot be grown in cell culture and enzyme immunoassays lack the necessary sensitivity, this technology is particularly useful.

Potential Commercial Applications: Competitive Advantages:
  • Development of norovirus diagnostics
  • Specific rtRT-PCR assay for detecting and distinguishing of the major pathogenic norovirus genogroups (I and II) within clinical and environmental samples
 
  • This is an internally controlled, multiplexed assay capable of rapid, accurate identification of norovirus genogroups responsible for human illness
  • Superior sensitivity compared with immunoassay detection methods


Inventors:
Jan Vinje (CDC)
Nicole Gregoricus (CDC)
Preeti Chhabra (CDC)
Leslie Barclay (CDC)
Hannah Shirley (CDC)
David Lee (CDC)


Intellectual Property:
US Application No. 61/560,077
PCT Application No. PCT/US2012/065269


Publications:
Vega E, et al. PMID 21801614
Schultz AC, et al. PMID 21195660

Licensing Contact:
Whitney Blair ,
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325
Rockville , MD 20852
Email: blairw2@mail.nih.gov
Phone: 301-435-4937
Fax: 301-402-0220

OTT Reference No: E-145-2013/0

Updated: 08/02/2013