Real-Time RT-PCR Assay for Detection of Noroviruses

A specific and sensitive TaqMan-based real-time (rt) RT-PCR assay has been developed by CDC scientists for detection of noroviruses in clinical and environmental specimens. This assay can be implemented to rapidly detect and distinguish norovirus strains from genogroups I and II, which are responsible for the majority of human infections. Additionally, the assay is multiplexed with an internal extraction control virus (coliphage MS2) to validate the results of the assay. Since the virus cannot be grown in cell culture and enzyme immunoassays lack the necessary sensitivity, this technology is particularly useful.

Potential Commercial Applications: Competitive Advantages:
  • Development of norovirus diagnostics
  • Specific rtRT-PCR assay for detecting and distinguishing of the major pathogenic norovirus genogroups (I and II) within clinical and environmental samples
  • This is an internally controlled, multiplexed assay capable of rapid, accurate identification of norovirus genogroups responsible for human illness
  • Superior sensitivity compared with immunoassay detection methods

Jan Vinje (CDC)
Nicole Gregoricus (CDC)
Preeti Chhabra (CDC)
Leslie Barclay (CDC)
Hannah Shirley (CDC)
David Lee (CDC)

Intellectual Property:
US Application No. 61/560,077
PCT Application No. PCT/US2012/065269

Vega E, et al. PMID 21801614
Schultz AC, et al. PMID 21195660

Licensing Contact:
Tara Kirby, Ph.D.
NIH Office of Technology Transfer
Phone: 301-435-4426

OTT Reference No: E-145-2013/0

Updated: Aug-02-2013