Real-Time RT-PCR Assay for Detection of Noroviruses
A specific and sensitive TaqMan-based real-time (rt) RT-PCR assay has been developed by CDC scientists for detection of noroviruses in clinical and environmental specimens. This assay can be implemented to rapidly detect and distinguish norovirus strains from genogroups I and II, which are responsible for the majority of human infections. Additionally, the assay is multiplexed with an internal extraction control virus (coliphage MS2) to validate the results of the assay. Since the virus cannot be grown in cell culture and enzyme immunoassays lack the necessary sensitivity, this technology is particularly useful.
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Jan Vinje (CDC)
Nicole Gregoricus (CDC)
Preeti Chhabra (CDC)
Leslie Barclay (CDC)
Hannah Shirley (CDC)
David Lee (CDC)
US Application No. 61/560,077
PCT Application No. PCT/US2012/065269
Vega E, et al. PMID 21801614
Schultz AC, et al. PMID 21195660
Tara Kirby, Ph.D.
NIH Office of Technology Transfer
OTT Reference No: E-145-2013/0