Rapid Molecular Assays for Specific Detection and Quantitation of Loa Loa Microfilaremia


The risk of fatal reactions in some infected individuals administered drug treatments for Loa loa infection, and the lack of accurate, convenient, diagnostics for this infection have thwarted efforts to eradicate the disease. Time consuming, labor intensive and training intensive microscope-based analysis of blood samples is the standard available diagnostic for Loa loa infection. This new assay technology introduces an easy to use, species-specific, highly sensitive, diagnostic that is able to be performed with minimal training. Positive test results may be indicated by an easily visualized color change and this test may be run without the need for expensive equipment such as a thermocycler. Because this test is rapid, cost efficient, labor efficient, accurate, and simple to run and read, it may be readily incorporated into portable point-of-care formats. These attributes make it ideally suited for use in locations where Loa loa infection is endemic. These advantages may lead to this technology becoming the new standard for diagnosis of Loa loa infections and a valuable tool, in control programs, to identify risks for adverse treatment reactions.

Potential Commercial Applications: Competitive Advantages:
  • Diagnostics testing
  • Infectious disease monitoring
  Greater speed cost and labor efficiencies, accurate, and simple to run and read and ability to be incorporated into portable point-of-care format, ideally suited for Loa loa endemic regions.


Inventors:
Thomas Nutman (NIAID)
Doran Fink (NIAID)


Intellectual Property:
PCT Application No. PCT/US2011/58565
US Application No. 61/410,232


Publications:
Fink DL, et al. doi:10.1371/journal.pntd.0001299
Klion AD, et al. PMID 2038361

Licensing Contact:
Edward (Tedd) Fenn ,
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325
Rockville , MD 20852
Email: fennea@mail.nih.gov
Phone: 301-435-5031
Fax: 301-402-0220

OTT Reference No: E-014-2011/0

Updated: 09/29/2011