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| Licensing & Royalties >> Licensing Opportunities >> Abstract Details |
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| C57BL/6J Embryonic Stem Cell Lines Generated Using Serum-Free Media |
Description of Invention:
NIH investigators have generated Embryonic Stem (ES) cell clones from C57BL/6J mice in a defined medium. These cell lines enable direct genetic alteration of mice in a pure genetic background.
Using a defined media supplement, knockout serum replacement (KSR) with knockout DMEM (KSR-KDMEM), the investigators established ES cell lines from blastocysts of C57BL/6J mice. One specific cell line, HGTC-8 was found to be karyotypically stable and germline competent, both prior to manipulation and after gene targeting. These cell lines transfected more efficiently, and exhibited increased efficiencies of cell cloning and chimera generation, when maintained in KSR-KDMEM.
Applications:
- Generation of knockout mice without the need to backcross.
- Generation of mice via targeted mutations.
Inventors:
Jun Cheng (NHGRI)
Pamela L Schwartzberg (NHGRI)
Patent Status:
HHS, Reference No. E-038-2009/0
Research Tool -- patent protection is not being pursued for this technology
Relevant Publication:
- J Cheng, A Dutra, A Takesono, L Garrett-Beal, PL Schwartzberg. Improved generation of C57BL/6J mouse embryonic stem cells in a defined serum-free media. Genesis. 2004 June; 39(2):100-104. [PubMed abs]
Licensing Status:
Available for licensing under a Biological Materials License Agreement.
For Licensing Information Please Contact:
Suryanarayana Vepa Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd. Suite 325,
Rockville, MD 20852
United States
Email: vepas@mail.nih.gov
Phone: 301-435-5020
Fax: 301-402-0220
Ref No: 1948
Updated: 05/2009
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